Engineering multiple levels of specificity in an RNA viral vector
Gao, X. J.; Chong, L. S.; Ince, M. H.; Kim, M. S.; Elowitz, M. B.
Show abstract
Synthetic molecular circuits could provide powerful therapeutic capabilities, but delivering them to specific cell types and controlling them remains challenging. An ideal “smart” viral delivery system would enable controlled release of viral vectors from “sender” cells, conditional entry into target cells based on cell-surface proteins, conditional replication specifically in target cells based on their intracellular protein content, and an evolutionarily robust system that allows viral elimination with drugs. Here, combining diverse technologies and components, including pseudotyping, engineered bridge proteins, degrons, and proteases, we demonstrate each of these control modes in a model system based on the rabies virus. This work shows how viral and protein engineering can enable delivery systems with multiple levels of control to maximize therapeutic specificity.Competing Interest StatementX.J.G, L.S.C., M.S.K., and M.B.E. are inventors on a U.S. patent provisional application related to this work.View Full Text
Matching journals
The top 2 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- HIV-1 binds dynein directly to hijack microtubule transport machinery 96%
- Elucidating the Mechanism by Which HIV-1 Nucleocapsid Mutations Confer Resistance to Integrase Strand Transfer Inhibitors 96%
- A conserved opal termination codon optimizes a temperature-dependent tradeoff between protein production and processing in alphaviruses 96%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.