How to quantitatively measure, assess and correct the fluorescence crosstalk in the wide-field microscopy
Tie, H. C.; Lu, L.
Show abstract
During the multi-color fluorescence imaging, a fluorophore can be detected in the non-corresponding channel due to the broad spectra of the fluorophore and filters, resulting in the artefact called the crosstalk (crossover or bleed-through). Unfortunately, the fluorescence crosstalk is ubiquitous for the conventional fluorescence microscopy. Significant crosstalk can distort the image and lead to incorrect interpretation. Here, we introduce a simple quantitative metric, the crosstalk factor, to measure the crosstalk effect of a fluorophore on a channel in the wide-field fluorescence microscopy. We describe a cell biologist friendly protocol which should be easily implemented by cell biologists using commonly available image processing software such as ImageJ.
Matching journals
The top 6 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- A framework to enhance the Signal-to-Noise Ratio for quantitative fluorescence microscopy 96%
- Probabilistic Optically-Selective Single-molecule Imaging Based Localization Encoded (POSSIBLE) Microscopy for Ultra-superresolution Imaging of Dendra2-HA transfected NIH3T3 cells 96%
- One-Shot phase-recovery using a Cellphone RGB Camera on a Jamin-Lebedeff Microscope 95%
Similar papers in this journal
- An evaluation of multi-excitation-wavelength standing-wave fluorescence microscopy (TartanSW) to improve sampling density in studies of the cell membrane and cytoskeleton 95%
- Stimulated emission depletion microscopy with a single depletion laser using five fluorochromes and fluorescence lifetime phasor separation 95%
- Light-Sheet Fluorescence Microscopy with Scanning Non-diffracting Beams 94%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.