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Silent dissemination of plasmid-borne tigecycline resistance gene tet(X6) in livestock-associated Acinetobacter towneri

Cheng, Y.; Chen, Y.; Liu, Y.; Song, J.; Guo, Y.; Zhou, Y.; Xiao, T.; Zhang, S.; Xu, H.; Chen, Y.-B.; Shan, T.; Xiao, Y.; Zhou, K.

2020-05-12 genetics
10.1101/2020.05.08.085514 bioRxiv
Show abstract

A tigecycline-resistance gene, tet(X6), was detected on a 159-kb novel plasmid carried by a tigecycline-susceptible livestock-associated Acinetobacter towneri isolate. The genetic context of tet(X6) ({Delta}ISVsa3-tet(X6)-abh-guaA-ISVsa3) is highly similar with that of the other plasmid-borne tet(X) variants. The 23-Ala residue of the first FAD binding site conferred higher activity to Tet(X6) than the 23-Gly reside conserved in the other plasmid-borne tet(X)s. To our knowledge, this is the first report of tet(X6) carried by the plasmid.

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