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A 5-min RNA preparation method for COVID-19 detection with RT-qPCR
Ladha, A.; Joung, J.; Abudayyeh, O.; Gootenberg, J.; Zhang, F.
2020-05-08
infectious diseases
10.1101/2020.05.07.20055947
medRxiv
Show abstract
RNA extraction has become a bottleneck for detection of COVID-19, in part because of reagent shortages. We present here a rapid protocol that circumvents the need for RNA extraction that is compatible with RT-qPCR-based detection methods.
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