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Myosin 10 and a Cytoneme-Localized Ligand Complex Promote Morphogen Transport

Hall, E. T.; Stewart, D. P.; Dillard, M.; Wagner, B.; Temirov, J.; Cheney, R. E.; Mori, M.; Robinson, C. G.; Ogden, S. K.

2020-05-07 cell biology
10.1101/2020.05.06.080820 bioRxiv
Show abstract

Morphogens function in concentration-dependent manners to instruct cell fate during tissue patterning. Molecular mechanisms by which these signaling gradients are established and reinforced remain enigmatic. The cytoneme transport model posits that specialized filopodia extend between morphogen-sending and responding cells to ensure that appropriate signal activation thresholds are achieved across developing tissues. How morphogens are transported along and deployed from cytonemes is not known. Herein we show that the actin motor Myosin 10 promotes cytoneme-based transport of Sonic Hedgehog (SHH) morphogen to filopodial tips, and that SHH movement within cytonemes occurs by vesicular transport. We demonstrate that cytoneme-mediated deposition of SHH onto receiving cells induces a rapid signal response, and that SHH cytonemes are promoted by a complex containing a ligand-specific deployment protein and associated co-receptor. One-Sentence summaryCytoneme-based delivery of the Sonic Hedgehog activation signal is promoted by Myosin 10 and BOC/CDON co-receptor function.

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