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SARS-CoV-2 genomes recovered by long amplicon tiling multiplex approach using nanopore sequencing and applicable to other sequencing platforms

Resende, P. C.; Motta, F. C.; Roy, S.; Appolinario, L.; Fabri, A.; Xavier, J.; Harris, K.; Matos, A. R.; Caetano, B. C.; Garcia, C. C.; Miranda, M. D.; Ogrzewalska, M.; Abreu, A.; Williams, R.; Breuer, J.; Siqueira, M. M.

2020-05-01 molecular biology
10.1101/2020.04.30.069039 bioRxiv
Show abstract

Genomic surveillance has become a useful tool for better understanding virus pathogenicity, origin and spread. Obtaining accurately assembled, complete viral genomes directly from clinical samples is still a challenging. Here, we describe three protocols using a unique primer set designed to recover long reads of SARS-CoV-2 directly from total RNA extracted from clinical samples. This protocol is useful, accessible and adaptable to laboratories with varying resources and access to distinct sequencing methods: Nanopore, Illumina and/or Sanger.

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