Titration of in-cellula affinities of protein-protein interactions
Cluet, D.; Vergier, B.; Levy, N.-P.; Dehau, L.; Thurman, A.; Amri, I.; Spichty, M.
Show abstract
A genetic assay permits simultaneous quantification of two interacting proteins and their bound fraction at the single-cell level using flow cytometry. In-cellula affinities of protein-protein interactions can be extracted from the acquired data through a titration-like analysis. The applicability of this approach is demonstrated on a diverse set of interactions with proteins from different families and organisms and with in-vitro dissociation constants ranging from picomolar to micromolar.
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