SARS-CoV-2 detection from nasopharyngeal swab samples without RNA extraction
Beltran-Pavez, C.; Marquez, C. L.; Munoz, G.; Valiente-Echeverria, F.; Gaggero, A.; Soto-Rifo, R.; Barriga, G. P.
Show abstract
The ongoing COVID-19 pandemic has reached more than 200 countries and territories worldwide. Given the large requirement of SARS-CoV-2 diagnosis and considering that RNA extraction kits are in short supply, we investigated whether two commercial RT-qPCR kits were compatible with direct SARS-CoV-2 detection from nasopharyngeal swab samples. We show that one of the tested kits is fully compatible with direct SARS-CoV-2 detection suggesting that omission of an RNA extraction step should be considered in SARS-CoV-2 diagnosis.
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