Back

A Self-Immobilizing NIR Probe for Non-invasive Imaging of Senescence

Liu, J.; Ma, X.; Cui, C.; Wang, Y.; Deenik, P. R.; Cui, L.

2020-03-29 biochemistry
10.1101/2020.03.27.010827 bioRxiv
Show abstract

Cellular senescence, a process that arrests the cell cycle, is a cellular stress response to various stimuli and is implicated in aging and age-related diseases. However, the understanding of senescence in living organisms is insufficient, largely due to the scarcity of sensitive tools for the detection of cellular senescence in vivo. Herein, we describe the development of a self-immobilizing near-infrared (NIR) probe that can be activated by senescence-associated {beta}- Galactosidase (SA-{beta}-Gal), a widely accepted senescence marker. The NIR fluorophore is turned on in the presence of SA-{beta}-Gal, and the self-immobilizing group, based on quinone methide chemistry, retains the fluorescence signal to the site of activation. This strategy significantly improves the sensitivity of the probe from the one we developed before. We demonstrate the non-invasive imaging of drug-induced senescence in mice models.

Matching journals

The top 16 journals account for 50% of the predicted probability mass.

50% of probability mass above

"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.