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Kentrophoros magnus sp. nov. (Ciliophora, Karyorelictea), a new flagship species of marine interstitial ciliates

Seah, B. K. B.; Volland, J.-M.; Leisch, N.; Schwaha, T.; Dubilier, N.; Gruber-Vodicka, H. R.

2020-03-20 microbiology
10.1101/2020.03.19.998534 bioRxiv
Show abstract

The karyorelictean ciliate Kentrophoros lacks a defined oral apparatus but has a dense coat of symbiotic bacteria that it consumes by phagocytosis. Body size, shape, and nuclear characters are variable in this genus. We formally describe a new species, K. magnus from Elba (Italy), which has unusual folding of its symbiont-bearing surface into pouch-like compartments, a body form that we term "pseudotrophosomal". K. magnus cells are large (2100 {+/-} 700 x 170 {+/-} 23 m in vivo), but contain only one micronucleus and two macronuclei, although these are much bigger than other Kentrophoros (widths 20 {+/-} 2.5 and 31 {+/-} 4.0 m respectively in K. magnus). We also present morphological observations on a close relative from Twin Cayes (Belize), which also has relatively large nuclei (micronuclei 13 {+/-} 1.5 m, mature macronuclei 20 {+/-} 2.8 m), but unlike K. magnus, it has on average 22 nuclei per cell, with different developmental stages of the macronuclei present simultaneously, and lacks pouch-like folding. Nuclear number and arrangement are important characters for karyorelicts. We suggest the use of a "nuclear formula" to simplify descriptions. Our discovery of large and morphologically distinctive new species underlines the incompleteness of our knowledge about meiofaunal ciliates.

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