Development of CRISPR as a prophylactic strategy to combat novel coronavirus and influenza
Abbott, T. R.; Dhamdhere, G.; Liu, Y.; Lin, X.; Goudy, L. E.; Zeng, L.; Chemparathy, A.; Chmura, S.; Heaton, N.; Debs, R.; Pande, T.; Endy, D.; La Russa, M.; Lewis, D. B.; Qi, L. S.
Show abstract
The outbreak of the coronavirus disease 2019 (COVID-19), caused by the Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2), has infected more than 100,000 people worldwide with over 3,000 deaths since December 2019. There is no cure for COVID-19 and the vaccine development is estimated to require 12-18 months. Here we demonstrate a CRISPR-Cas13-based strategy, PAC-MAN (Prophylactic Antiviral CRISPR in huMAN cells), for viral inhibition that can effectively degrade SARS-CoV-2 sequences and live influenza A virus (IAV) genome in human lung epithelial cells. We designed and screened a group of CRISPR RNAs (crRNAs) targeting conserved viral regions and identified functional crRNAs for cleaving SARS-CoV-2. The approach is effective in reducing respiratory cell viral replication for H1N1 IAV. Our bioinformatic analysis showed a group of only six crRNAs can target more than 90% of all coronaviruses. The PAC-MAN approach is potentially a rapidly implementable pan-coronavirus strategy to deal with emerging pandemic strains.
Matching journals
The top 5 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Reprogrammed CRISPR-Cas13b suppresses SARS-CoV-2 replication and circumvents its mutational escape through mismatch tolerance 98%
- Improved prime editors enable pathogenic allele correction and cancer modelling in adult mice 96%
- A conserved long-range RNA interaction in SARS-CoV-2 recruits ADAR1 to enhance virus proliferation 96%
Similar papers in this journal
- HIV-1 Vpr induces ciTRAN to prevent transcriptional silencing of the provirus 96%
- Pharmacological modulators of epithelial immunity uncovered by synthetic genetic tracing of SARS-CoV-2 infection responses 96%
- A conserved opal termination codon optimizes a temperature-dependent tradeoff between protein production and processing in alphaviruses 95%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.