Fluorophore-labelled RNA aptamers to common protein tags as super-resolution imaging reagents.
Wang, J.; Singh, A.; Ozer, A.; Zipfel, W. R.
Show abstract
Developing labelling methods that densely and specifically label targeted cellular structures is critically important for centroid localization-based super-resolution microscopy. Being easy and inexpensive to produce in the laboratory and of relatively small size, RNA aptamers have potential as a substitute for conventional antibody labelling. By using aptamers selected against common protein tags - GFP (green fluorescent protein) in this case - we demonstrate labelling methods using dSTORM-compatible fluorophores for STORM and hybridizable imager strands for DNA-PAINT super-resolution optical imaging of any cellular proteins fused to the aptamer binding target. We show that we can label both extracellular and intracellular proteins for super-resolution imaging, and that the method in particular, offers some interesting advantages for live cell super-resolution imaging of plasma membrane proteins. KEY POINTSO_LIA simple to use RNA aptamer method for super-resolution STORM and PAINT imaging in cells expressing common protein tags. C_LIO_LIThe method has a number of unique advantages for live cell imaging at the nanometer scale. C_LIO_LIProvides a means to quantify the number of proteins being trafficked on the plasma membrane. C_LI
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