CRISPR-based Live Imaging of Epigenetic Modification-Mediated Genome Reorganization
Feng, Y.; Wang, Y.; Yang, C.; Naseri, A.; Pederson, T.; Zheng, J.; Xiao, X.; Zhang, S.; Xie, W.; Ma, H.
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Epigenetic modifications play an essential role in chromatin architecture and dynamics. The role of epigenetic modification in chromatin organization has been studied by Hi-C from population cells, but imaging techniques to study their correlation and regulation in single living cells are lacking. Here we develop a CRISPR-based EpiGo (Epigenetic perturbation induced Genome organization) system to track epigenetic modification-mediated relocation, interaction or reorganization of genomic regions in living cells. EpiGo-KRAB is sufficient to induce the relocation of genomic loci to HP1 condensates and trigger genomic interactions. EpiGo-KRAB also triggers the induction of H3K9me3 at large genomic regions, which decorate on the surface of HP1 condensates possibly driven by phase separation.
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