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How does group differences in motion scrubbing affect false positives in functional connectivity studies?

Eklund, A.; Nichols, T.; Afyouni, S.; Craddock, C.

2020-02-12 neuroscience
10.1101/2020.02.12.944454 bioRxiv
Show abstract

Analyzing resting state fMRI data is difficult due to a weak signal and several noise sources. Head motion is also a major problem and it is common to apply motion scrubbing, i.e. to remove time points where a subject has moved more than some pre-defined motion threshold. A problem arises if one cohort on average moves more than another, since the remaining temporal degrees of freedom are then different for the two groups. The effect of this is that the uncertainty of the functional connectivity estimates (e.g. Pearson correlations) are different for the two groups, but this is seldom modelled in resting state fMRI. We demonstrate that group differences in motion scrubbing can result in inflated false positives, depending on how the temporal auto correlation is modelled when performing the Fisher r-to-z transform.

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