Tandem tension sensor reveals substrate rigidity-dependence of integrin molecular tensions in live cells
Sarkar, A.; LeVine, D.; Zhao, Y.; Mollaeian, K.; Ren, J.; Wang, X.
Show abstract
Response of integrin tensions to substrate rigidity is important in cell rigidity sensing but has not been confirmed. Current fluorescent tension sensors produce cellular force signals collectively resulted from integrin tension magnitude, tension dwell time, integrin density and activity, ligand density and accessibility, etc., making it challenging to monitor the absolute molecular force level of integrin tensions in live cells. Here we developed a tandem tension sensor (TTS) consisting of two coupled tension sensing units which are subject to the same tension and respond with different activation probabilities to the tension. Reported by fluorescence, the activation probability ratio of these two units solely responds to the force level of local integrin tensions, excluding the bias from other non-force factors. We verified the feasibility of TTS in detecting integrin tensions and applied it to study cells on elastic substrates. TTS unambiguously reported that integrin tensions in platelets decrease monotonically with the substrate rigidity, verifying the rigidity-dependence of integrin tensions in live cells.
Matching journals
The top 13 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Cost-efficient boundary-free surface patterning achieves high effective-throughput of time-lapse microscopy experiments 95%
- Rapid fabrication of hydrogel micropatterns by projection stereolithography for studying self-organized developmental patterning 95%
- Evaluating sources of technical variability in the mechano-node-pore sensing pipeline and their effect on the reproducibility of single-cell mechanical phenotyping 95%
Similar papers in this journal
- The nuclear lamin network passively responds to both active or passive cell movement through confinements 93%
- Cell adhesion strength and tractions are mechano-diagnostic features of cellular invasiveness 93%
- Active microrheology using pulsed optical tweezers to probe viscoelasticity of Lamin A towards diagnosis of laminopathies 92%
Similar papers in this journal
- Long-term label-free assessments of individual bacteria using three-dimensional quantitative phase imaging and hydrogel-based immobilization 95%
- Unique Cancer Motility Behaviors in Confined Spaces of Microgroove Topography with Acute Wall Angles 95%
- Controlled tumor heterogeneity in a co-culture system by 3D bio-printed tumor-on-chip model 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.