Novel culture method to diagnose fungal infections with biopsy tissues
Zhao, J.; Zhang, Y.; Fan, Y.; Xiong, Z.; Xia, Y.; Zou, X.; Han, J.; Li, B. B.; Wang, C.; Liu, X.; Lu, B.; Cao, B.
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Biopsy tissue is a difficult sample to gain for the identification of potential pathogen in the clinical laboratory. At present, there are no effective culture methods for small-sized biopsy tissue. In this study, we propose a novel tissue biopsy culture method based on Myco/F lytic liquid culture system, namely, tissue was grinded before injected into culture bottles. More types and numbers of fungi are cultivated using this culture system than sputum or BALF culture. A few of mycobacteria were also detected with ground tissues. The method may be a promising alternative to or supplement for the traditional plate culture in clinical practice.
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