Germ plasm localisation dynamics mark distinct phases of transcriptional and post-transcriptional regulation control in primordial germ cells
D'Orazio, F. M.; Balwierz, P.; Guo, Y.; Rodriguez, B. H.; Jasiulewicz, A.; Vaquerizas, J. M.; Cairns, B.; Lenhard, B.; Mueller, F.
Show abstract
In many animal models, primordial germ cell (PGC) development depends on maternally-deposited germ plasm to avoid somatic cell fate. Here, we show that PGCs respond to regulatory information from the germ plasm in two distinct phases and mechanisms in zebrafish. We show that PGCs commence zygotic genome activation together with the rest of the embryo with no demonstrable differences in transcriptional and chromatin accessibility levels. Thus, cytoplasmic germ plasm determinants only affect post-transcriptional stabilisation of RNAs to diverge transcriptome from somatic cells, which, unexpectedly, also activate germ cell-specific genes. Perinuclear relocalisation of germ plasm is coupled to dramatic divergence in chromatin opening and transcriptome from somatic cells characterised by PGC-specific chromatin topology. Furthermore, we reveal Tdrd7, regulator of germ plasm localisation, as crucial determinant of germ fate acquisition.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Characterising open chromatin identifies novel cis-regulatory elements important for paraxial mesoderm formation and axis extension 97%
- Activator-blocker model of transcriptional regulation by pioneer-like factors 96%
- Rapid redistribution and extensive binding of NANOG and GATA6 at shared regulatory elements underlie specification of divergent cell fates 96%
Similar papers in this journal
- Establishment of 3D chromatin structure after fertilization and the metabolic switch at the morula-to-blastocyst transition require CTCF 97%
- Orchestration of pluripotent stem cell genome reactivation during mitotic exit 95%
- SLAMseq resolves the kinetics of maternal and zygotic gene expression in early zebrafish embryogenesis 94%
Similar papers in this journal
- The miR-430 locus with extreme promoter density is a transcription body organizer, which facilitates long range regulation in zygotic genome activation 96%
- Developmental cell fate choice employs two distinct cis regulatory strategies 96%
- Structural perturbation of chromatin domains with multiple developmental regulators can severely impact gene regulation and development 96%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.