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Tiled Reconstruction Improves Structured Illumination Microscopy

Hoffman, D. P.; Betzig, E.

2020-01-06 cell biology
10.1101/2020.01.06.895318 bioRxiv
Show abstract

Structured illumination microscopy (SIM) is widely used for fast, long-term, live-cell super-resolution imaging. However, SIM images can contain substantial artifacts if the sample does not conform to the underlying assumptions of the reconstruction algorithm. Here we describe a simple, easy to implement, process that can be combined with any reconstruction algorithm to alleviate many common SIM reconstruction artifacts and briefly discuss possible extensions.

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