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Developmental regulation of oocyte lipid uptake via 'patent' tricellular junctions in Drosophila epithelium

Row, S.; Deng, W.-M.

2020-01-01 developmental biology
10.1101/2019.12.31.891838 bioRxiv
Show abstract

Epithelia form protective permeability barriers that selectively allow the exchange of material while maintaining tissue integrity under extreme mechanical, chemical, and bacterial loads. Here, we report in the Drosophila follicular epithelium a developmentally regulated and evolutionarily conserved process, patency, wherein a breach is created in the epithelium at tricellular contacts during mid-vitellogenesis. In Drosophila, patency exhibits a strict temporal range delimited by the transcription factor Tramtrack69, and a spatial pattern regulated by the dorsal-anterior signals of the follicular epithelium. Crucial for lipid uptake by the oocyte, patency is also exploited by endosymbionts such as Spiroplasma pulsonii. Our findings reveal an evolutionarily conserved non-typical epithelial function in a classic model system.

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