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UP{varphi} phages, a new group of filamentous phages found in several members of Enterobacteriales

Shapiro, J. W.; Putonti, C.

2019-12-28 microbiology
10.1101/2019.12.27.889675 bioRxiv
Show abstract

Filamentous phages establish chronic infections in their bacterial hosts, and new phages are secreted by infected bacteria for multiple generations, typically without causing host death. Often, these viruses integrate in their hosts genome by co-opting the hosts XerCD recombinase system. In several cases, these viruses also encode genes that increase bacterial virulence in plants and animals. Here, we describe a new filamentous phage, UP{phi}901, which we originally found integrated in a clinical isolate of uropathogenic Escherichia coli. UP{phi}901 and closely related phages can be found in published genomes of over 200 other bacteria, including strains of Citrobacter koseri, Salmonella enterica, Yersinia enterocolitica, and Klebsiella pneumoniae. Its closest relatives are consistently found in urine or in the blood and feces of patients with urinary tract infections. More distant relatives can be found in isolates from other environments, including sewage, water, soil, and contaminated food. Each of these phages, which we collectively call "UP{phi} viruses," also harbors two novel genes of unknown function.

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