Visualizing the metazoan proliferation-differentiation decision in vivo
Kohrman, A. Q.; Adikes, R. C.; Smith, J. J.; Martinez, M. A. Q.; Medwig-Kinney, T. N.; Palmisano, N. J.; Sallee, M. D.; Ahmed, O. B.; Weeks, N.; Kim, N.; Liu, S.; Zhang, W.; Pani, A. M.; Matus, D. Q.
Show abstract
Cell proliferation and terminal differentiation are intimately coordinated during metazoan development. Here, we adapt a cyclin-dependent kinase (CDK) sensor to uncouple these cell cycle-associated events live in C. elegans and zebrafish. The CDK sensor consists of a fluorescently tagged CDK substrate that steadily translocates from the nucleus to the cytoplasm in response to increasing CDK activity and consequent sensor phosphorylation. We show that the CDK sensor can distinguish cycling cells in G1 from terminally differentiated cells in G0, revealing a commitment point and a cryptic stochasticity in an otherwise invariant C. elegans cell lineage. We also derive a predictive model of future proliferation behavior in C. elegans and zebrafish based on a snapshot of CDK activity in newly born cells. Thus, we introduce a live-cell imaging tool to facilitate in vivo studies of cell cycle control in a wide-range of developmental contexts.
Matching journals
The top 2 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Physically asymmetric division of the C. elegans zygote ensures invariably successful embryogenesis 97%
- Dynamic compartmentalization of the pro-invasive transcription factor NHR-67 reveals a role for Groucho in regulating a proliferative-invasive cellular switch in C. elegans 97%
- Stereotyped behavioral maturation and rhythmic quiescence in C. elegans embryos 97%
Similar papers in this journal
- Evolutionarily conserved midbody reorganization precedes ring canal formation during gametogenesis 95%
- Accelerated cell cycles enable organ regeneration under developmental time constraints in the Drosophila hindgut 95%
- Hippo pathway and Bonus control developmental cell fate decisions in the Drosophila eye 95%
Similar papers in this journal
- Pre-dauer starvation rapidly and reversibly reduces niche proliferative signaling to the C. elegans germ line 97%
- FGF diffusion is required for directed migration of postembryonic muscle progenitors in C. elegans 96%
- Dendrites with specialized glial attachments develop by retrograde extension using SAX-7 and GRDN-1 96%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.