Systems genetics analysis identify calcium signalling defects as novel cause of congenital heart disease
Izarzugaza, J. M. G.; Ellesoe, S. G.; Doganli, C.; Ehlers, N. S.; Dalgaard, M. D.; Audain, E.; Dombrowsky, G.; Sifrim, A.; Wilsdon, A.; Thienpont, B.; Breckpot, J.; Gewillig, M.; Competence Network for Congenital Heart Defects, Germany, ; Brook, J. D.; Hitz, M.-P.; Larsen, L. A.; Brunak, S.
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BackgroundCongenital heart disease (CHD) occurs in almost 1% of newborn children and is considered a multifactorial disorder. CHD may segregate in families due to significant contribution of genetic factors in the disease aetiology. The aim of the study was to identify pathophysiological mechanisms in families segregating CHD. MethodsWe used whole exome sequencing to identify rare genetic variants in ninety consenting participants from 32 Danish families with recurrent CHD. We applied a systems biology approach to identify developmental mechanisms influenced by accumulation of rare variants. We used an independent cohort of 714 CHD cases and 4922 controls for replication and performed functional investigations using zebrafish as in vivo model. ResultsWe identified 1,785 genes, in which rare alleles were shared between affected individuals within a family. These genes were enriched for known cardiac developmental genes and 218 of the genes were mutated in more than one family. Our analysis revealed a functional cluster, enriched for proteins with a known participation in calcium signalling. Replication confirmed increased mutation burden of calcium-signalling genes in CHD patients. Functional investigation of zebrafish orthologues of ITPR1, PLCB2 and ADCY2 verified a role in cardiac development and suggests a combinatorial effect of inactivation of these genes. ConclusionsThe study identifies abnormal calcium signalling as a novel pathophysiological mechanism in human CHD and confirms the complex genetic architecture underlying CHD.
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