Laboratory Evaluation of Cyclospora cayetanensis in Wastewater: Expanding Pathogen Targets for Regulatory Public Health Surveillance
Lin, C.-Y.; Gonzalez, D. S.; Poretsky, R. S.
Show abstract
Wastewater-based epidemiology (WBE) has become a cornerstone of public health surveillance, yet routine regulatory monitoring remains largely focused on respiratory viruses and selecting antimicrobial resistant bacteria. Emerging foodborne parasites, such as Cyclospora cayetanensis, remain excluded from standard environmental surveillance panels despite increasing domestic outbreaks. This study presents a laboratory-based pilot protocol evaluating the feasibility of extracting and quantifying C. cayetanensis from urban wastewater across the Chicago metropolitan area. Using sample concentration via centrifugation and mechanical bead-beating, automated magnetic bead nucleic acid extraction, and digital PCR (dPCR), we demonstrate high-sensitivity detection in municipal influents and sewer nodes. These findings establish a proof-of-concept laboratory methodology to support the expansion of regulatory public health surveillance frameworks to include non-routine protozoan pathogens.
Matching journals
The top 6 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- High-resolution within-sewer SARS-CoV-2 surveillance facilitates informed intervention 96%
- SARS-CoV-2 RNA titers in wastewater anticipated COVID-19 occurrence in a low prevalence area 95%
- Wastewater Monitoring of SARS-CoV-2 from Acute Care Hospitals Identifies Nosocomial Transmission and Outbreaks 95%
Similar papers in this journal
- Bio-Rad and QIAGEN digital PCR platforms provide equivalent quantification for wastewater-based SARS-CoV-2 surveillance 98%
- Source-to-Tap Investigation of the Occurrence of Nontuberculous Mycobacteria in a Full-Scale Chloraminated Drinking Water System 96%
- Development of wastewater pooled surveillance of SARS-CoV-2 from congregate living settings 96%
Similar papers in this journal
- Simultaneous detection and quantification of multiple pathogen targets in wastewater 96%
- Environmental surveillance of soil-transmitted helminths and other enteric pathogens in settings without networked wastewater infrastructure 96%
- Operationalizing a routine wastewater monitoring laboratory for SARS-CoV-2 95%
Similar papers in this journal
- Longitudinal wastewater-based surveillance of vancomycin-resistant Enterococci in tertiary-care hospitals 96%
- Detection of SARS-CoV-2 in wastewater in Japan by multiple molecular assays-implication for wastewater-based epidemiology (WBE) 96%
- First Detection Of SARS-COV-2 In Untreated Wastewaters In Italy 96%
Similar papers in this journal
- SARS-CoV-2 in wastewater settled solids is associated with COVID-19 cases in a large urban sewershed 96%
- Flushing of stagnant premise water systems after theCOVID-19 shutdown can reduce infection risk byLegionella and Mycobacterium spp. 96%
- Solid evidence and liquid gold: trade-offs of processing settled solids, whole influent, or centrifuged influent for co-detecting viral, bacterial, and eukaryotic pathogens in wastewater 95%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.