Isolation and characterisation of Lytic Jumbo Klebsiella phage BMPCR_005026
Lloyd-Evans, I.; Ford, M.; Hazzard, J.; Bartis, C.; Patel, F.; Roemer, N.; Wright, K.; Porter, K.; Coleman, M.; Galyov, E.; Megremis, S.; Clokie, M. R.; Millard, A. D.; Michniewski, S.; Wilkinson, R. C.
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Background: Klebsiella pneumoniae is a key causative agent of multidrug resistant nosocomial infections. Bacteriophages offer a promising alternative treatment, but the diversity of Klebsiella species requires the characterisation of a broad range of phages, particularly the poorly characterised jumbo phages. Materials and Methods: Phage BMCPR_005026 was isolated from sewage against Klebsiella pneumoniae. Whole genome sequencing, transmission electron microscopy and host range analysis against a diverse set of 40 clinically relevant Klebsiella strains were used to characterise the phage. Results: The virion had a myovirus morphology and genomic analysis placed it within an undefined family in Caudoviricetes where it forms a new species within the genus Alcyoneusvirus. BMCPR_005026 could form plaques on 3 of the 40 Klebsiella strains tested (7.5%) and formed halos. Conclusion: BMCPR_005026 represents a new species of phage within the genus Alcyoneusvirus with activity against 3 Klebsiella KL-types.
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