Scalable generation of human stem cell-derived ureteric bud and collecting duct organoids in stirred bioreactors
Wolf, K. J.; Rubins, J.; van Gaal, R.; Stevens, K.; Klaus, A.; Nair, P.; Lewis, J. A.
Show abstract
Emerging efforts to engineer multicellular kidney tissues for drug discovery, disease modeling, and therapeutic repair require scalable generation of kidney organoids derived from human induced pluripotent stem cells (hiPSCs). Here, we report a method for generating kidney ureteric bud and collecting duct epithelial organoids in stirred tank bioreactors. We demonstrate that extracellular matrix microcarriers facilitate organoid expansion and are compatible with suspension bioreactor methods. The combination of cell-coated matrix microcarriers with stirred bioreactors promotes maturation marker expression compared to static controls comprising matrix-embedded organoids. Our method offers promise for generating epithelial organoids that serve as multicellular models and organ building blocks for scalable biofabrication.
Matching journals
The top 5 journals account for 50% of the predicted probability mass.
Similar papers in this journal
Similar papers in this journal
- Paracrine Factor Local Gradient-Generating System for Engineering Perfusable Vascularized Hepatocyte Tissues with Perfusion-Induced Proliferation 95%
- A Pillar and Perfusion Plate Platform for Robust Human Organoid Culture and Analysis 95%
- Democratizing Organ-on-Chip Technologies with a Modular, Reusable, and Perfusion-Ready Microphysiological System 94%
Similar papers in this journal
- Topographic Guidance in Melt-Electrowritten Tubular Scaffolds Enhances Engineered Kidney Tubule Performance 94%
- Enhanced microvasculature formation and patterning in iPSC-derived kidney organoids cultured in physiological hypoxia 93%
- Recapitulation of human embryonic heart beating to promote differentiation of hepatic endoderm to hepatoblasts 92%
Similar papers in this journal
- A dialysis medium refreshment cell culture set-up for an osteoblast-osteoclast coculture 91%
- Characterization of three-dimensional bone-like tissue growth and organization under influence of curvature and directional fluid flow 90%
- Controlling human stem cell-derived islet composition using magnetic sorting 89%
Similar papers in this journal
- Human lung organoids develop into adult airway-like structures directed by physico-chemical biomaterial properties 94%
- Thiol-ene cross-linked alginate hydrogel encapsulation modulates the extracellular matrix of kidney organoids by reducing abnormal type 1a1 collagen deposition 94%
- A novel Organ-Chip system emulates three-dimensional architecture of the human epithelia and allows fine control of mechanical forces acting on it. 94%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.