Cultivation and Sequencing Reveal Nutrient-Dependent Bacterial Responses in Public Restrooms
Weng, J.; Ying, B.-W.
Show abstract
Microbial communities in indoor environments are shaped by resource availability and disturbances, yet their growth dynamics and compositional changes remain unclear. Here we combined quantitative colony growth analysis with 16S rRNA gene sequencing to investigate bacterial communities on public restroom surfaces before and after routine cleaning under varied nutrient conditions. Cultivation revealed that nutrient availability strongly influenced bacterial growth and selectively enriched distinct taxa, while cleaning caused limited shifts in overall community structure and diversity. Correlations between growth parameters and diversity indices were weak, indicating that taxon-specific responses to nutrients primarily drive growth outcomes. These findings suggest that resource composition, rather than cleaning disturbance, governs bacterial growth and community assembly in built environments. Integrating culture-based phenotyping with sequencing provides a comprehensive framework to understand microbial dynamics following environmental perturbations.
Matching journals
The top 6 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Disturbance-Diversity Relationships of Microbial Communities Change Based on Growth Substrate 93%
- Interactions Between Native Soil Microbiome and a Synthetic Microbial Community Reveals Bacteria with Persistent Traits 93%
- Microbial consortiums of putative degraders of low-density polyethylene-associated compounds in the ocean 93%
Similar papers in this journal
Similar papers in this journal
Similar papers in this journal
- Soil properties in agricultural systems affect microbial genomic traits 93%
- Lactic Acid Bacteria Dominate Urban Bokashi: A culture-independent study of Microbial Diversity and Functional Potential in Household-Scale Food Waste Fermentation 92%
- A Machine Learning Approach Elucidates Spatial Patterns of Environmental Properties Driving Microbial Composition Over Santos Basin, South Atlantic 91%
Similar papers in this journal
- Microbial Similarity between Students in a Common Dormitory Environment Reveals the Forensic Potential of Individual Microbial Signatures 96%
- Ecological and genomic attributes of novel bacterial taxa that thrive in subsurface soil horizons 94%
- High-Density Microdroplet Cultivation Reveals the Essential Role of Microbial Interactions in the Growth of Environmental Microbes 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.