Mitotic catastrophe and other cellular instability events in sodium valproate-treated HeLa cells
Sforca, B. P.; Oliveira, C. B.; Furtado, M. M.; Santos, M. G.; Rocha, M. A.; Mello, M. L. S.
Show abstract
Valproic acid/sodium valproate (VPA) is a widely prescribed anticonvulsant and has also been used against certain tumor cells. It is a potent modulator of gene expression. Its ability to induce apoptosis has been well documented in HeLa cells. However, another form of cell death - mitotic catastrophe - has not yet been explored in VPA-treated HeLa cells. Here, we investigated the effects of VPA treatment on mitotic catastrophe characteristics, including morphological features and their frequencies, fluorescence intensity signals of caspase-2 and p53, and the expression and abundance of DNMT1 and DNMT3B. An increased frequency of mitotic catastrophe was observed not only morphologically, but also through enhanced induction of caspase-2, involvement of p53, at least under more drastic VPA treatment, but without a decrease in DNMT1 or DNMT3B levels. Additionally, enhancement of mitotic catastrophe coincided with a reduction in mitotic chromosome abnormalities. Increased DNMT3B expression following VPA action, may be favored by previously reported chromatin decondensation induced by this drug. Enhanced CpG methylation of specific DNA sites could thus be promoted. In conclusion, VPA was shown to trigger metabolic pathways linked to different forms of cell death in HeLa cells, supporting its oncosuppressive potential.
Matching journals
The top 10 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Antitumor Activities and Cellular Changes Induced by TrkB Inhibition in Medulloblastoma 94%
- The mitochondrial deubiquitinase USP30 regulates AKT/mTOR signaling 93%
- Molecular mechanism of action of repurposed drugs and traditional Chinese medicine used for the treatment of patients infected with COVID-19: A systematic scoping review 91%
Similar papers in this journal
- The biotoxin BMAA promotes dysfunction via distinct mechanisms in neuroblastoma and glioblastoma cells 94%
- A Priori Activation of Apoptosis Pathways of Tumor (AAAPT) Technology: Development of Targeted Apoptosis Initiators for Cancer Treatment. 93%
- Application of non-invasive low-intensity pulsed electric field with thermal cycling-hyperthermia for synergistically enhanced anticancer effect of chlorogenic acid on PANC-1 cells 93%
Similar papers in this journal
- Facilitation and inhibition of firing activity and N-methyl-D-aspartate-evoked responses of CA1 hippocampal pyramidal cells by alpha7 nicotinic acetylcholine receptor selective compounds in vivo 94%
- The combination of lipopolysaccharide and D-galactosamine administration show positive genotoxic effect in mice liver 94%
- Cannabidiol (CBD) as a novel inhibitor of HLA-G expression in human choriocarcinoma cell line (JEG-3) 93%
Similar papers in this journal
- Thermal cycling-hyperthermia attenuates rotenone-induced cell injury in SH-SY5Y cells through heat-activated mechanisms 93%
- Protein profiling of WERI RB1 and etoposide resistant WERI ETOR reveals new insights into topoisomerase inhibitor resistance in retinoblastoma 92%
- A small molecule that promotes cellular senescence prevents fibrogenesis and tumorigenesis in vitro 92%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.