Comparative methods for iPSC-Derived endothelial cells in modeling vascular diseases.
Akkaya, P. N.; Koolen, L.; Hosseinzadeh, Z.
Show abstract
Endothelial cells (ECs) derived from human induced pluripotent stem cells (hiPSCs) are increasingly used to model vascular diseases and test therapeutic strategies. However, the efficiency and reproducibility of differentiation can vary depending on the culture medium and its supplemented factors and stages. Here, we directly compared two defined media, APEL and BPEL, for iPSC-to-ECs differentiation. iPSCs were differentiated over 10 days with sequential growth factor induction, followed by magnetic-activated cell sorting or flow cytometry for CD31+ cells. Both media produced ECs with similar morphology and marker expression, including CD31 and VE-cadherin. Functional assays demonstrated comparable tube formation, indicating equivalent endothelial functionality. Cost analysis indicated that APEL had a higher total reagent cost but generated a higher total cell yield, resulting in a comparable cost per 10 total cells, whereas BPEL was more cost-efficient for producing CD31/VE-cadherin endothelial-specific cells. Our results suggest that APEL and BPEL media are equally effective for generating iPSC-derived ECs, providing flexibility in method selection for vascular disease modeling and drug discovery applications.
Matching journals
The top 10 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Isolation of adipose tissue derived regenerative cells from human subcutaneous tissue with or without the use of an enzymatic reagent 93%
- Mesenchymal Stem/ Stromal Cells metabolomic and bioactive factors profiles: a comparative analysis on the Umbilical Cord and Dental Pulp derived Stem/ Stromal Cells secretome 93%
- Effects of amotosalen treatment on human platelet lysate bioactivity 93%
Similar papers in this journal
- Animal-Origin-Free Method for Generating Blood Vessel Organoids 97%
- Investigating the role of molecular coating in human corneal endothelial cell primary culture using artificial intelligence-driven image analysis 94%
- TRAP seq in 3D Angiogenesis Assays Reveals a Distinct Endothelial Translatome Associated with Early and Late Stages of Morphogenesis. 92%
Similar papers in this journal
- Substrate elasticity does not impact on DNA methylation changes during differentiation of pluripotent stem cells 91%
- Single-cell microencapsulation improves lung retention of endothelial colony forming cells after intravascular delivery and unmasks therapeutic benefit in severe pulmonary arterial hypertension 91%
- Accessory-cell-free differentiation of hematopoietic stem and progenitor cells into mature red blood cells 90%
Similar papers in this journal
- Uniform cerebral organoid culture on a pillar plate by simple and reproducible spheroid transfer from an ultralow attachment well plate 93%
- 3D bioprinting of co-cultured osteogenic spheroids for bone tissue fabrication 92%
- Dynamic culture of cerebral organoids using a pillar/perfusion plate for the assessment of developmental neurotoxicity 91%
Similar papers in this journal
- Recapitulation of human embryonic heart beating to promote differentiation of hepatic endoderm to hepatoblasts 91%
- Enhanced microvasculature formation and patterning in iPSC-derived kidney organoids cultured in physiological hypoxia 90%
- Methods for Processing and Analyzing Images of Vascularized Micro-Organ and Tumor Systems 90%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.