Back

High thoughput fluorometric nucleic acid quantification using qPCR instruments

Meerson, A.

2026-08-06 molecular biology
10.64898/2026.08.01.742208 bioRxiv
Show abstract

To explore adapting qPCR systems for end-point nucleic acid quantification using dyes such as SYTO-9, we quantified serial dilutions of DNA and RNA standards in the range of 0.75 - 200 ng/{micro}l on 384-well qPCR devices. SYTO-9 fluorescence was successfully measured using standard SYBR Green settings. Blank-subtracted relative SYTO-9 signal showed a logarithmic dependence on DNA/RNA concentration (R2 > 0.95). Measurements were highly stable with different incubation times, temperatures of up to 95{degrees}C, and photobleaching. The described approach is a valuable QC option for high-throughput DNA/RNA isolations and could be adapted to additional fluorometric assays beyond nucleic acids.

Matching journals

The top 7 journals account for 50% of the predicted probability mass.

1
PLOS ONE
● 5266 papers in training set
Top 9%
19.1%
Similar papers in this journal
2
Scientific Reports
○ 3612 papers in training set
Top 6%
8.2%
Similar papers in this journal
3
Journal of Virological Methods
○ 37 papers in training set
Top 0.1%
5.7%
Similar papers in this journal
4
Talanta
○ 14 papers in training set
Top 0.1%
5.7%
Similar papers in this journal
5
Biology Methods and Protocols
◐ 61 papers in training set
Top 0.1%
5.0%
Similar papers in this journal
6
Analytical Chemistry
● 218 papers in training set
Top 0.8%
4.2%
7
Journal of Microbiological Methods
○ 13 papers in training set
Top 0.1%
3.3%
50% of probability mass above
8
Nucleic Acids Research
◐ 1281 papers in training set
Top 6%
2.9%
9
Molecular Ecology Resources
○ 171 papers in training set
Top 0.8%
2.7%
10
Forensic Science International: Genetics
○ 26 papers in training set
Top 0.1%
2.2%
11
Methods
○ 34 papers in training set
Top 0.2%
1.8%
12
SLAS Discovery
○ 25 papers in training set
Top 0.1%
1.8%
13
Current Protocols
○ 15 papers in training set
Top 0.2%
1.6%
14
Journal of Clinical Microbiology
● 130 papers in training set
Top 0.9%
1.5%
15
BioTechniques
○ 25 papers in training set
Top 0.2%
1.5%
16
MethodsX
○ 16 papers in training set
Top 0.1%
1.4%
17
STAR Protocols
○ 18 papers in training set
Top 0.2%
1.1%
18
Nature Communications
○ 5641 papers in training set
Top 52%
1.1%
19
Environmental DNA
○ 56 papers in training set
Top 0.6%
1.0%
20
Genes
○ 144 papers in training set
Top 3%
0.9%
21
Applied and Environmental Microbiology
● 339 papers in training set
Top 5%
0.9%
22
Viruses
○ 332 papers in training set
Top 4%
0.9%
23
The Journal of Molecular Diagnostics
○ 39 papers in training set
Top 0.6%
0.9%
24
Clinical Chemistry
◐ 22 papers in training set
Top 0.4%
0.6%
25
Communications Biology
○ 993 papers in training set
Top 33%
0.6%
26
International Journal of Infectious Diseases
○ 129 papers in training set
Top 3%
0.6%
27
BMC Methods
○ 15 papers in training set
Top 0.2%
0.6%
28
RNA
● 189 papers in training set
Top 1%
0.6%
29
Frontiers in Bioengineering and Biotechnology
○ 98 papers in training set
Top 3%
0.6%
30
ACS Synthetic Biology
● 287 papers in training set
Top 2%
0.6%

"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.