Enhancing Recombinant Vector Assembly Efficiency: A Novel Methodological Approach
YUAN, S.; Jiang, H.; Wang, H.; Fu, M.; Wang, J.; Liu, Z.; Li, Y.
Show abstract
With the rapid development of modern biotechnology, DNA vectors have become fundamental tools for inserting, transferring, and expressing specific gene sequences in various fields such as gene cloning, gene expression, gene editing, and gene therapy. However, when dealing with complex structured DNA sequences, traditional vector construction methods face challenges with low connection efficiency. This study proposes a new method for constructing recombinant vectors by employing a strategy of high-temperature treatment followed immediately by placement on ice, effectively reducing the complexity of DNA structures and enhancing the efficiency of PCR product-vector connection, thereby improving the construction efficiency of recombinant vectors. This paper describes the technical details of the method, experimental validation, and applications in gene cloning, gene recombination editing, and the preparation of gene therapy drugs, providing a new efficient tool for molecular biology experiments.
Matching journals
The top 12 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- RecombiCraft Library construction: A novel method for DNA Library cloning and expansion using non-enzymatic single-step DNA recombination and liquid culture 95%
- In silico analysis and in planta production of recombinant ccl21/IL1β protein and characterization of its in vitro anti-tumor and immunogenic activity 93%
- Akaby - cell-free protein expression system for linear templates 93%
Similar papers in this journal
- Converting Escherichia coli MG1655 into a chemical overproducer through inactivating defense system against exogenous DNA 93%
- Highly efficient genome editing in Bacillus subtilis via miniature DNA nucleases IscB. 93%
- Simplified Methodology for a Modular and Genetically Expanded Protein Synthesis in Cell-Free Systems 92%
Similar papers in this journal
- Enhancing RNA Delivery: Practical Insights into NeoLNP Transfection Reagent 92%
- RevGraphVAMP: A protein molecular simulation analysis model combining graph convolutional neural networks and physical constraints 89%
- Penguin: A Tool for Predicting Pseudouridine Sites in Direct RNA Nanopore Sequencing Data 89%
Similar papers in this journal
Similar papers in this journal
- Simple cloning of large natural product biosynthetic gene clusters from Streptomyces by an engineered CRISPR/Cas12a system 95%
- Targeted mRNA demethylation using an engineered dCas13b-ALKBH5 fusion protein 92%
- RaptRanker: in silico RNA aptamer selection from HT-SELEX experiment based on local sequence and structure information 91%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.