Identification, optimization, and structural elucidation of chloroacetamide scaffold as covalent inhibitors for Ubiquitin C-terminal Hydrolase L3
Beeralingappa, N. C.; Lu, M.; Patel, R.; Pannala, N.; Dhiman, A.; Heil, B. N.; Imhoff, R. D.; Smith, E. G.; Bahler, M. B.; Marsden, H. L.; Allen-Petersen, B. L.; Wendt, M. K.; Das, C.; Flaherty, D. P.
Show abstract
The deubiquitinating enzyme, ubiquitin C-terminal hydrolase L3 (UCHL3), has been implicated as a potential therapeutic target for cancer with a role in regulating the DNA damage response pathways. While the target has been studied using genetic methods there is a lack of reliable chemical probes to selectivity target UCHL3. In this study we report hit identification and optimization of a new chemical scaffold that irreversibly inhibits UCHL3. The observed structure-activity relationships are corroborated by ligand-bound crystal structures that confirm covalent adduct formation with the catalytic cysteine of the enzyme. Finally, through gel-shift assays using a ubiquitin activity-based probe we demonstrate on-target engagement with UCHL3 in two cell lines. The work as a whole presents a comprehensive evaluation of the new scaffold that can be utilized to probe UCHL3 in different biological contexts.
Matching journals
The top 3 journals account for 50% of the predicted probability mass.