Back
Droplet-compatible single-cell DNA methylation sequencing with PreTIC
Zhang, S.; Wang, F.; Zhang, Y.; Lee, K.-J.; Engman, C.; He, H.-Z.; Fan, Y.; Zheng, S.-Y.
2026-04-18
genomics
10.64898/2026.04.15.718726
bioRxiv
Show abstract
Single-cell DNA methylation sequencing has remained technically specialized due to challenges of interfacing conversion chemistry with droplet microfluidics. We introduce pre-tagmentation in situ conversion (PreTIC), which enables whole-methylome profiling on a commercial droplet platform using off-the-shelf reagents. With PreTIC, we produce over 13,000 single-cell methylomes from fixed frozen cells in two days and resolve cell-specific epigenetic variation in human peripheral blood mononuclear cells.
Matching journals
●Non-profit
◐University press
○Commercial
The top 2 journals account for 50% of the predicted probability mass.
1
Nature Biotechnology
○
147 papers in training set
Top 0.1%
27.3%
2
Nature Communications
○
4913 papers in training set
Top 2%
23.8%
50% of probability mass above
3
Nature Methods
○
336 papers in training set
Top 1%
8.9%
4
Cell
○
370 papers in training set
Top 8%
2.7%
5
Cell Reports Methods
○
141 papers in training set
Top 1%
2.2%
6
Nucleic Acids Research
◐
1128 papers in training set
Top 9%
2.0%
7
Genome Medicine
○
154 papers in training set
Top 4%
2.0%
8
Science
●
429 papers in training set
Top 13%
1.9%
9
Nature Biomedical Engineering
○
42 papers in training set
Top 0.8%
1.8%
10
Communications Biology
○
886 papers in training set
Top 10%
1.6%
11
Genome Biology
○
555 papers in training set
Top 5%
1.6%
12
Small Methods
○
26 papers in training set
Top 0.6%
1.3%
13
Science Advances
●
1098 papers in training set
Top 22%
1.3%
14
Cell Genomics
○
162 papers in training set
Top 4%
1.3%
15
ACS Nano
●
99 papers in training set
Top 3%
0.9%
16
Nature Machine Intelligence
○
61 papers in training set
Top 3%
0.9%
17
Advanced Science
○
249 papers in training set
Top 16%
0.9%
18
Nature
○
575 papers in training set
Top 15%
0.8%
19
Advanced Materials
○
53 papers in training set
Top 2%
0.8%
20
Scientific Reports
○
3102 papers in training set
Top 74%
0.8%
21
Genome Research
●
409 papers in training set
Top 5%
0.7%
22
PLOS ONE
●
4510 papers in training set
Top 70%
0.7%
23
Circulation
○
66 papers in training set
Top 3%
0.5%
24
Proceedings of the National Academy of Sciences
●
2130 papers in training set
Top 48%
0.5%