Introducing the digital PCR data essentials standard to harmonize data structure for clinical and research use
Trypsteen, W.; Vynck, M.; Untergrasser, A.; Whale, A. S.; Rodiger, S.; Dobnik, D.; Bogozalec Kosir, A.; Milavec, M.; Kubista, M.; Pfaffl, M. W.; Nour, A. A.; Young-Kyung, B.; Bustin, S. A.; Calin, G.; Chen, Y.; Cleveland, M. H.; De Falco, A.; Forootan, A.; O'Sullivan, D. M.; Devonshire, A. S.; Foy, C. A.; Fraley, S. I.; Gleerup, D. G.; He, H.-J.; Hellemans, J.; Lievens, A.; Lind, G. E.; Porco, D.; Romsos, E. L.; Thas, O.; Drandi, D.; de Tayrac, M.; Taly, V.; Huggett, J. F.; Vandesompele, J.; De Spiegelaere, W.
Show abstract
Digital PCR (dPCR) is a powerful technology for absolute quantification of nucleic acids, valued for its accuracy, sensitivity, and repeatability. Yet, the commercialization of different instruments with proprietary software has introduced challenges to data analysis, interoperability, and comparability. Therefore, we present the Digital PCR Data Essentials Standard (DDES) - a lightweight, human- and machine-readable, and cross-platform data standard developed in collaboration with the dPCR community. The standard consists of three file types designed to enable both manual inspection and automated analysis: (i) a main file summarizing experiment and reaction-level (meta-)data; (ii) an assay file describing targets and detection chemistry, and (iii) intensity files capturing partition-level raw fluorescence data per reaction. DDES supports a wide range of current dPCR applications, including singleplex and multiplex assays, endpoint and real-time readouts, and will be curated to implement future dPCR developments. By harmonizing the data structure, DDES lays out the foundation for FAIR dPCR data practices and supports improved software compatibility, collaborative and reproducible research, and future dPCR data repositories.
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