Characterization of Nanoparticles in Suspension by Simultaneous iNTA and Fluorescence Detection with Single-Molecule Sensitivity
Jiang, S.; Kashkanova, A. D.; Lee, H.; Miller, M. E. C.; Utikal, T.; Shkarin, A.; Qazvini, H.; Sandoghdar, V.
Show abstract
Quantitative knowledge of nanoparticle properties is desirable in a large number of scientific and technological applications, but measurements with a high degree of precision usually prove to be challenging. Among a range of available methodologies, optical techniques with single particle sensitivity are especially interesting because they can reveal intrinsic hetero-geneities in a fast non-invasive manner. Recently, we presented interferometric nanoparticle tracking analysis (iNTA) as a highly sensitive label-free technique that is capable of determining the size, concentration and index of refraction of different subpopulations in a suspension mixture. Here, we enhance this method with biochemical specificity through multicolor fluorescence detection at the single-molecule sensitivity limit. We benchmark the performance of the combined technique, which we name iNTA-F, by distinguishing populations of fluorescent and non-fluorescent nanoparticles of different material, size, and fluorescence intensity, with an emphasis on the characterization of lipid vesicles and biological extracellular vesicles (EVs).
Matching journals
The top 10 journals account for 50% of the predicted probability mass.