An AI-Assisted Workflow for Reconstruction, Extension, and Calibration of Quantitative Systems Pharmacology Models.

AbstractsO_ST_ABSBackgroundC_ST_ABSQuantitative systems pharmacology (QSP) models provide mechanistic insight into drug response but are limited by labor-intensive, expert-driven workflows. We developed an AI-assisted QSP (AI-QSP) framework that integrates large language models (LLMs) with SBML-based modeling to enable automated reconstruction, extension, and calibration of mechanistic models. MethodsThe framework was applied to a published CAR-T QSP model. The model was reconstructed in SBML and extended via LLM-guided prompts to incorporate key resistance mechanisms: T-cell exhaustion, PD-1/PD-L1 checkpoint regulation, and tumor antigen escape. Model development followed an iterative expert-in-the-loop workflow. The resulting model (21 reactions, 9 species) was calibrated to synthetic benchmark data using 19-parameter optimization. Model credibility was assessed using ASME V&V 40 and ICH M15 principles, including global sensitivity and profile-likelihood analyses. ResultsThe calibrated model reproduced benchmark dynamics with high accuracy (mean log-RMSE = 0.132). Sensitivity analysis identified CAR-T killing and bystander cytotoxicity as dominant drivers of tumor response. Profile-likelihood analysis showed 71% of parameters were practically identifiable, with remaining parameters prioritised for future data-driven refinement. ConclusionsAI-assisted QSP modeling enables reproducible, scalable model reconstruction and evolution while maintaining mechanistic transparency and regulatory alignment. This framework provides a foundation for accelerating model-informed drug development in cell and gene therapies.