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NHAPL analysis of glycoRNA reveals sialic acid-containing glycosylated mRNA 3UTRs and enables sensitive SLE diagnostics
2026-02-09
rheumatology
Title + abstract only
View on medRxiv
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GlycoRNA, newly identified RNA molecules bearing glycan modifications on cell membranes, is implicated in cell communication and immune regulation. However, current technological limitations impede a thorough elucidation of their biological roles and clinical significance. Here, we developed Nucleotides Hybridization and Aptamer-based Proximity Ligation (NHAPL), a homogeneous assay enabling sensitive and quantitative glycoRNA analysis from 160pg total cell RNA and 1{micro}l serum. NHAPL integrat...
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