Deep immunophenotyping reveals associations with human blood pressure

Preclinical models have been instrumental in defining the immunological mechanisms underlying hypertension, yet human studies remain comparatively limited, leaving a major gap in translational understanding. To address this, we investigated immune cell dynamics in human hypertension by profiling peripheral blood mononuclear cells from 48 normotensive and hypertensive participants, characterised using ambulatory blood pressure (BP) monitoring. High{square}dimensional spectral flow cytometry revealed strong positive correlations between night{square}time systolic BP and multiple CD8{square}T{square}cell subsets. Hypertensive participants also exhibited significantly elevated CD8{square}T cell subsets, a pattern that persisted even among those receiving anti{square}hypertensive medication with subsequently controlled BP. These findings suggest that BP control does not necessarily equate to biological control of hypertension and highlight a distinct CD8{square}T cell-driven signature that differentiates individuals with normal BP and hypertension.