Use of Metagenomic Reference Materials to Compare Lysis and Extraction Methods Against a Known Input
Weinstein, M. M.; Tierney, B. T.; Wolfe, E. Y.; Tang, S.; Jacobs, J. P.; Lagishetty, V.; Kemp, R.; Mason, C.
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Inconsistent cellular lysis is a major source of inaccuracy in microbial analysis; there is a need to develop objective and standardized evaluation of lysis efficiency across diverse methods. Using a mock microbial community as a ground truth, we built a bioinformatic and experimental toolkit for this purpose, comparing the performance of chemical, thermal, enzymatic, and various physical lysis protocols. We evaluated over 150 lysis conditions and observed the highest performance from mechanically- and materially-optimized bead-based approaches. To extend these findings to a practical setting, we demonstrated that the Firmicutes to Bacteroides ratio, a frequently used compositional metric in gut microbiome analysis is biased heavily by insufficient lysis, indicating the importance of benchmarking ongoing methodological development.
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