Angle-resolved Measurements Reveal the Origin of Signal Anisotropy in Pump-probe Microscopy
Jia, X.; Warren, W. S.
Show abstract
Nonlinear optical microscopy modalities have become widely used in applications ranging from material characterization to tissue diagnosis. In complex systems, multiple mechanisms often contribute to the signal; for example, two-color pump-probe microscopy provides rich molecular signatures of the samples based on several nonlinear processes. Here we uncover a surprising complication, that in many cases the detection direction drastically alters the signal components and the overall amplitude. To understand the origin of this signal anisotropy, we first calculate and measure a simple case, the pump-probe signal of gold nanoshells at various detection angles, attributing the effect to scattering changes due to the sample refractive index change; we then extend this analysis, showing a striking directional dependence arising from distinct scattering profiles of two dominant nonlinear processes. These results demonstrate that comparing epi (backward) and transmission (forward) signals provides additional information, enabling cleaner separation of nonlinear contributions in pump-probe measurements.
Matching journals
The top 11 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Wide-field bond-selective fluorescence imaging: from single-molecule to cellular imaging beyond video-rate 95%
- Circumventing the optical diffraction limit with customized speckles 95%
- Tandem RAman Microscopy (TRAM): integration of spontaneous and coherent Raman scattering offering data fusion analysis to improve optical biosensing 95%
Similar papers in this journal
- mEos4b photoconversion efficiency depends on laser illumination conditions used in PALM 94%
- Ratiometric Sensing of Redox Environments Inside Individual Carboxysomes Trapped in Solution 92%
- High efficiency excitation energy transfer in biohybrid quantum dot-bacterial reaction center nanoconjugates 92%
Similar papers in this journal
- Real-time precision opto-control of chemical processes in live cells 96%
- Exfoliated near infrared fluorescent CaCuSi4O10 nanosheets with ultra-high photostability and brightness for biological imaging 95%
- Mitochondrial dynamics quantitatively revealed by STED nanoscopy with an enhanced squaraine variant probe 95%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.