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Molecular differentiation by PCR of the Entamoeba dispar/histolytica/moshkovskii complex in the population of Coyaima, Tolima, Colombia

2025-12-17 infectious diseases Title + abstract only
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The Entamoeba histolytica/dispar/moshkovskii complex comprises morphologically indistinguishable species, making accurate diagnosis difficult and leading to overestimations in amebiasis prevalence. This study aimed to establish a sensitive and specific molecular method to differentiate these species in human fecal samples from Coyaima, Tolima, Colombia. A polymerase chain reaction (PCR) targeting the small subunit ribosomal RNA (18S rRNA) gene was developed and optimized under various amplificat...

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