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Alginate Nanohydroxyapatite Composite for Dental Tissue Engineering Application.

Nayak, A. N.; Ramachandran, R.; Shah, H. P.; Chowdhary, I.; Sharma, P.

2025-12-15 bioengineering
10.64898/2025.12.11.693830 bioRxiv
Show abstract

Traumatic dental tissue injury is a common health concern globally, with more than a billion people affected worldwide. In this study, we have synthesized green route nanohydroxyapatite utilizing green tea extract, which further enhances the osteogenesis, osteointegration, and biocompatibility of the synthesized hydroxyapatite. This Nanohydroxyapatite was capped with an antibiotic, integrated into an alginate scaffold, and coated with a painkiller to create a dual drug release system, providing rapid release of painkiller and sustained release of antibacterial drug. Biocompatibility and osteoblast proliferation were confirmed through MTT assays, and osteogenesis by ALP activity, validating the suitability of the scaffold for dental bone tissue engineering applications. The developed nanocomposite scaffold shows significant promise in enhancing dental bone repair by simultaneously addressing the inflammation, infection, and regeneration challenges. Graphical Abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=129 SRC="FIGDIR/small/693830v1_ufig1.gif" ALT="Figure 1"> View larger version (27K): org.highwire.dtl.DTLVardef@124b53forg.highwire.dtl.DTLVardef@f75868org.highwire.dtl.DTLVardef@cd20a6org.highwire.dtl.DTLVardef@11a7dfb_HPS_FORMAT_FIGEXP M_FIG C_FIG

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