Prophage Abundance Differentiates Clinical and Environmental Isolates of Pseudomonas aeruginosa
Targ, R. W.; Blankenberg, P. M.; Pourtois, J. D.; Burgener, E. B.; Milla, C. E.; Bollyky, P. L.
Show abstract
The bacterium Pseudomonas aeruginosa can grow in soil and water environments as well as the human body, where it is an opportunistic pathogen. Here, we have investigated how the number and integrity of prophages present in the P. aeruginosa reflects these adaptations. We analyzed genomic sequences from 139 environmental and 145 clinical P. aeruginosa isolates. We find that clinical genomes are smaller (6.5{+/-}0.29 Mbp) than environmental genomes (6.7{+/-}0.29 Mbp, p = 0.002), and exhibit higher GC content (66.25{+/-}0.2% vs. 65.32{+/-}0.3%). Genome size is inversely correlated with GC content, with clinical isolates having both smaller genome size and increased GC content. Much of the genome size difference is attributable to mobile genetic elements. Environmental isolates had more and longer intact prophages (12.33 in comparison to a clinical average of 11.5, though this finding was not statistically significant (Welchs t-test p=0.31)), and accumulated many fragmented prophage remnants. These do not appear to be lineage-associated effects - phylogenetic analyses support that both transitions from clinical to environmental and environmental to clinical occur. Our findings suggest that reduced genome size and loss of intact prophages are hallmark of the specialization of P. aeruginosa to the human body.
Matching journals
The top 5 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Exploring the mobilome and resistome of Enterococcus faecium in a One Health context across two continents 94%
- Large scale and significant expression from pseudogenes in Sodalis glossinidius - a facultative bacterial endosymbiont 94%
- Genomics of Irish swine-derived Streptococcus suis: Population structure, prophages, and anti-viral defence mechanisms 94%
Similar papers in this journal
- A Genome-based Model to Predict the Virulence of Pseudomonas aeruginosa Isolates 95%
- Genomic surveillance of vancomycin-resistant Enterococcus faecium reveals spread of a linear plasmid conferring a nutrient utilization advantage 94%
- Genomic adaptation in group B Streptococcus following intrapartum antibiotic prophylaxis and childbirth 94%
Similar papers in this journal
Similar papers in this journal
- Species-scale genomic analysis of S. aureus genes influencing phage host range and their relationships to virulence and antibiotic resistance genes 94%
- Genomic diversity of hospital-acquired infections revealed through prospective whole genome sequencing-based surveillance 94%
- Genomic characterization of the C. tuberculostearicum species complex, a ubiquitous member of the human skin microbiome 94%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.