Back

HiFi sequencing accurately identifies clinically relevant variants in paralogous genes

2025-10-31 genetic and genomic medicine Title + abstract only
View on medRxiv
Show abstract

Short-read sequencing (SRS) methods have improved the detection of small genetic variants but remain limited in highly homologous genomic regions, such as segmental duplications with gene-pseudogene pairs. These paralogous regions often require complex, locus-specific assays for accurate analysis. Long-read genome sequencing (lrGS) technologies, such as PacBio HiFi sequencing, can span these regions but still face challenges in variant calling due to alignment ambiguities. Here, we evaluated Pac...

Predicted journal destinations