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Prodan-based solvatochromic probes for polarity imaging of organelles

Aknine, N.; Holban, P.; Ragaller, F.; Carravilla, P.; Gurdap, C. O.; Cetinkaya, A.; Sezgin, E.; Klymchenko, A. S.

2025-10-17 biophysics
10.1101/2025.10.17.683001 bioRxiv
Show abstract

Solvatochromic probes provide microscopic, structural and functional information on their targeted cellular compartments. In this field, the challenge lies in designing probes that are both sufficiently sensitive to environment, and specific in their subcellular localization. Here, we design Prodan-based polarity probes targeting the following organelles: mitochondria, endoplasmic reticulum, Golgi apparatus, lysosomes and lipid droplets. The new probes provide robust organelle targeting, except for the mitochondrial probe, whose targeting ability is cell type dependent. Due to operating range of Prodan fluorophore in the UV-Blue region, our probes can be easily combined with other fluorescent tags in the visible range. Therefore, polarity of sub-cellular compartments can be studied together with additional fluorescent reporters. We used these probes to show the polarity of organelle membranes in healthy cells and under starvation condition where we observed organelle-specific polarity remodeling. These probes are important addition to the repertoire of smart cellular probes and will find critical use in understanding spatiotemporal regulation of cellular physiology.

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