Wireless Colorimetric Multi-Biomarker Sensing to Enable Critical Neonatal Monitoring

Clinical monitoring in the most vulnerable patients such as newborns relies on invasive and costly procedures and/or wired sensor surveillance, increasing discomfort and risk for undetected events. Addressing this critical need, we present a noninvasive, biocompatible silk-based sensor capturing multiple critical body functions via colorimetric analysis of body fluids, optimized for its use in critically ill preterm neonates where transepidermal fluid mirrors the interstitial compartment, thereby opening new avenues for future clinical monitoring. The sensor introduces twelve different colorimetric inks that stabilize labile bioresponsive molecules to a simple, wax-printed paper microfluidic wearable patch to facilitate real-time tracking of biomarkers (temperature (T), pH, sodium (S), and glucose (G)) on a miniaturized surface. Colorimetric responses showed high precision and reproducibility in sensing ranges relevant for neonatal care (T: 32-41 [{degrees}C]; pH: 3-9; S: 2.92-29.20 [mg/mL], G: 0.039-0.625 [mg/mL]). Deep learning for color response quantification achieved automated measurement (T:0.455 [{degrees}C]; pH:0.416; S:0.857 [mg/mL], G:0.019 [mg/mL]; mean absolute error). The sensors optimized interface for sample collection on skin and its performance under clinically relevant conditions, e.g., increased humidity (80%), tracking on moving object (0.986 AP @ IoU=0.5), sensor shear/rotation, uncontrolled light, as well as successful capture of physiological effects in biological fluids together with its miniaturized design caters to the critical needs of intensified monitoring in high-end patient populations such as neonates. Significance StatementWe designed a noninvasive, wearable and miniaturized paper sensor patch capturing critical body functions via colorimetric analysis of body fluids (perspiration and saliva) adapted to the clinical needs of neonatal monitoring. Through twelve different bioresponsive inks stabilized in silk-fibroin into a simple, wax-printed paper microfluidic patch, the sensor facilitates simultaneous real-time tracking of multiple biomarkers under clinically relevant conditions using deep learning models for automated sensor detection and parameter estimation. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=185 SRC="FIGDIR/small/25336258v1_ufig1.gif" ALT="Figure 1"> View larger version (48K): org.highwire.dtl.DTLVardef@dafa2corg.highwire.dtl.DTLVardef@5e9db7org.highwire.dtl.DTLVardef@1c4795eorg.highwire.dtl.DTLVardef@1fc25e5_HPS_FORMAT_FIGEXP M_FIG Graphical Abstract C_FIG