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Query Augmented Generation (QAG) from the Genomic DataCommons for Accurate Variant Statistics

Venkat, A.; Wysocki, W. P.; Lukowski, M.; Song, S.; Subramanyam, A.; Zhang, Z.; Grossman, R. L.

2025-09-07 bioinformatics
10.1101/2025.09.02.673797 bioRxiv
Show abstract

In precision oncology, researchers often use public knowledgebases to check somatic variant frequencies against their cohort data. Large language models (LLMs) can quickly answer questions on somatic variant frequencies, but often hallucinate and give inaccurate results for factual data. Using synthetic queries, we show that somatic variant frequencies in baseline LLM responses are underestimated compared to the Genomic Data Commons (GDC), the worlds largest data commons for cancer research. We present a modular architecture called Query Augmented Generation (QAG) for integrating LLMs with high-quality data from a third party data source such as a data commons, knowledgebase or database. We apply QAG to the GDC to help researchers obtain accurate frequencies for somatic variants, copy number variants, and MSI status--even for complex queries requiring multiple steps in the GDC portal and API. Our software is deployed as a model context protocol (MCP) server on Hugging Face and available on GitHub.

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