A Validated LC-MS/MS Method for Quantifying Phenolic Acids, Lignans, and Enterolignans from Human Fecal Samples
Dicksion, C. A.; Chao, D. N.; Rickmeyer, J. D.; Bess, E. N.
Show abstract
The human gut microbiome is home to numerous small molecules that impact health. Three prominent classes of molecules in this environment include phenolic acids, lignans, and enterolignans, which have been linked to anti-inflammatory and anti-oxidant effects as well as protection from cancer, cardiovascular disease, and neurodegeneration. The abundance of these molecules in the gut microbiome as well as their biological significance motivated the development of the LC-MS/MS method reported herein, which provides a simple, robust, and high-throughput approach to simultaneously quantify a 17-membered panel of phenolic acids, lignans, and enterolignans in human fecal microbiome samples. This method employs liquid-liquid extraction, which allows for cost-effective sample preparation by using common laboratory materials. Additionally, fecal samples are lyophilized prior to extraction to mitigate confounding effects introduced by variability in water content that exists across individuals samples. Inclusion of 0.1% acetic acid in the chromatographic solvents optimized peak shape and signal intensity. Plastic microcentrifuge tubes imparted substantial interferences for some analytes, which was resolved through use of glass vials. Using this method, 60% of human fecal samples from 10 individuals showed that phenolic acids bearing a catechol motif were in significantly greater abundance than were guaiacols. As specific gut bacteria can transform guaiacols into redox-active catechols, determination of guaiacol:catechol ratios in fecal samples may afford a biomarker of gut microbiota anti-oxidant potential. For the Table of Contents only O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=106 SRC="FIGDIR/small/673557v1_ufig1.gif" ALT="Figure 1"> View larger version (29K): org.highwire.dtl.DTLVardef@11985a6org.highwire.dtl.DTLVardef@955d08org.highwire.dtl.DTLVardef@ee39aorg.highwire.dtl.DTLVardef@180a0d3_HPS_FORMAT_FIGEXP M_FIG C_FIG
Matching journals
The top 6 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- UCL-MetIsoLib: A Public High-Resolution Tandem Mass Spectrometry Library for HILIC-Based Isomer-Resolved Profiling of Glycolysis, Central Carbon Metabolism, and Beyond in Urine, Plasma, Tissues, Cells, and Patient-Derived Organoids 96%
- Data-independent acquisition coupled with electron-activated dissociation for in-depth structure elucidation of fatty acid ester of hydroxy fatty acids 96%
- Profiling Active Enzymes for Polysorbate Degradation in Biotherapeutics by Activity-Based Protein Profiling 94%
Similar papers in this journal
- Systematic analysis of in-source modifications of primary metabolites during flow-injection time-of-flight mass spectrometry 92%
- Spatially resolved distribution of pancreatic hormones proteoforms by MALDI-imaging mass spectrometry 92%
- Development and validation of an HPLC method to quantify 2-Keto-3-deoxy-gluconate (KDG), a major metabolite in pectin and alginate degradation pathways 90%
Similar papers in this journal
- High-throughput UHPLC-MS to screen metabolites in feces for gut metabolic health 97%
- An Optimised Monophasic Faecal Extraction Method for LC-MS Analysis and its Application in Gastrointestinal Disease 96%
- Optimized Mass Spectrometry Detection of Thyroid Hormones and Polar Metabolites in Rodent Cerebrospinal Fluid 95%
Similar papers in this journal
- Development, validation and application of an LC-MS/MS method quantifying free forms of the micronutrients queuine and queuosine in human plasma using a surrogate matrix approach 96%
- Maternal PFAS Transfer through Lactation: Dolphin Milk Reveals Routes of Early-Life Exposure 95%
- A highly sensitive LC-MS/MS method for quantitative determination of 7 vitamin D metabolites in mouse brain tissue 93%
Similar papers in this journal
- A combined flow injection/reversed phase chromatography - high resolution mass spectrometry workflow for accurate absolute lipid quantification with 13C- internal standards 94%
- In-situ lipid profiling of insect pheromone glands by Direct Analysis in Real Time Mass Spectrometry 93%
- A candidate reference method for the quantification of α-synuclein in cerebrospinal fluid using an SI traceable primary calibrator and multiple reaction monitoring 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.