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Comment on "Inverted repeats in the monkeypox virus genome are hot spots for mutation"

Madorskaya, A. V.; Kazanov, F. M.; Ponomarev, G. V.; Smolnikova, A.; Garshina, D.; Matveev, E.; Abasov, R. K.; Ivankov, D. N.; Gelfand, M. S.; Kazanov, M. D.

2025-08-20 microbiology
10.1101/2025.08.19.670320 bioRxiv
Show abstract

Recent work proposed that APOBEC-signature mutations in monkeypox virus (MPXV) are enriched in cruci-form structures formed by inverted repeats. We independently reanalyzed the same dataset with the published settings and several complementary approaches and found no statistically supported enrichment of mutations in these structures. While a modest trend at TC motif at 3 end of hairpin loop contexts cannot be excluded, current evidence is insufficient. Additional data and careful handling of overlapping structures are needed to clarify any association.

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