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An open, integrated platform for multiplexed bioluminescence microscopy

Scipioni, L.; Torrado, B.; Tedeschi, G.; Halbers, L.; Torrey, Z. R.; Fuller, E. B.; Fersini, F.; Gohlke, C.; Luptak, A.; Prescher, J. A.; Digman, M. A.

2025-08-22 bioengineering
10.1101/2025.08.17.670750 bioRxiv
Show abstract

We report an imaging package to democratize all-in-one bioluminescence and fluorescence microscopy. The platform comprises three tools: PhasorViewer, a visualization suite to design experiments and identify optimal probe combinations; PhasorScope, an open-source, cost-effective microscopy framework to upgrade conventional microscopes; and PhasorAnalysis, a dedicated, user-friendly analysis pipeline to quantify phasor imaging data. We demonstrate the utility of the platform for multiplexed, simultaneous fluorescence and bioluminescence imaging with readily accessible optical reporters.

Published in Chemical & Biomedical Imaging · not in our set (fewer than 10 published preprints to learn from) · training set

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"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.