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Rakaia: interactive discovery of spatial biology at scale

Watson, M.; Abazari, G.; Chen, E. L.; Tan, T. J.; Gorman, J. L.; Diorio, C.; Berman, H. K.; Campbell, K. R.; Jackson, H. W.

2025-08-20 bioinformatics
10.1101/2025.08.15.670550 bioRxiv
Show abstract

Spatial biology data throughput currently outpaces interpretable analysis, limiting large-scale discovery and translation. Here we present Rakaia, a browser-based platform for multiplexed imaging and spatial transcriptomics, empowering code-free interactive exploration and evaluation. Rakaia enables visualization, annotation, and feature-based querying with prioritization across thousands of images. We use Rakaia to identify cell types of interest in >200 highly multiplexed images from non-malignant human breast tissue. Rakaia is available at: https://rakaia.io/

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